Harper Adams University Repository

Methods for genomic characterization and maintenance of anaerobic fungi

Peng, X., Swift, C.L., Theodorou, M.K. and O'Malley, M.A. (2018) Methods for genomic characterization and maintenance of anaerobic fungi. In: Fungal genomics: methods and protocols. Springer, New York, pp. 53-67.

[img] Text
Mike Theodorou methods upload.pdf - Accepted Version
Restricted to Repository staff only until 7 June 2020.

Download (257kB) | Request a copy


The rapid development of molecular biology and bioinformatics has fueled renewed interests in anaerobic fungi from the phylum Neocallimastigomycota. This chapter presents well-established methods for isolation, routine cultivation, and cryopreservation of anaerobic fungi. Moreover, detailed nucleic acid extraction protocols are provided, which should enable readers to isolate high-quality DNA and RNA from a variety of anaerobic fungal culture media for downstream applications such as next-generation sequencing.

Item Type: Book Section
Keywords: Anaerobic fungi, Genomes, Transcriptomes, DNA extraction, RNA extraction, Cultivation, Isolatio, n Neocallimastigomycota, Next-generation sequencing, Consecutive batch culture
Divisions: Animal Production, Welfare and Veterinary Sciences
Depositing User: Ms Kath Osborn
Date Deposited: 08 Jul 2019 13:27
Last Modified: 08 Jul 2019 13:27
URI: http://hau.collections.crest.ac.uk/id/eprint/17424

Actions (login required)

Edit Item Edit Item