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Methods for genomic characterization and maintenance of anaerobic fungi

Peng, X., Swift, C.L., Theodorou, M.K. and O'Malley, M.A. (2018) Methods for genomic characterization and maintenance of anaerobic fungi. In: Fungal genomics: methods and protocols. Springer, New York, pp. 53-67.

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Abstract

The rapid development of molecular biology and bioinformatics has fueled renewed interests in anaerobic fungi from the phylum Neocallimastigomycota. This chapter presents well-established methods for isolation, routine cultivation, and cryopreservation of anaerobic fungi. Moreover, detailed nucleic acid extraction protocols are provided, which should enable readers to isolate high-quality DNA and RNA from a variety of anaerobic fungal culture media for downstream applications such as next-generation sequencing.

Item Type: Book Section
Keywords: Anaerobic fungi, Genomes, Transcriptomes, DNA extraction, RNA extraction, Cultivation, Isolatio, n Neocallimastigomycota, Next-generation sequencing, Consecutive batch culture
Divisions: Animal Production, Welfare and Veterinary Sciences
Depositing User: Ms Kath Osborn
Date Deposited: 08 Jul 2019 13:27
Last Modified: 08 Jul 2019 13:27
URI: http://hau.collections.crest.ac.uk/id/eprint/17424

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